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Test Code N.CDIFF C. DIFFICILE TOXIN A/B  (Screen)

Methodology

TECHLAB rapid membrane enzyme immunoassay for the detection of Clostridioides (Clostridium) difficile toxins A and B in stool specimens. This test is useful for the rapid diagnosis of Clostridium difficile associated diarrhea (CDAB) and pseudomembranous colitis.

Specimen Requirements

 Soft or Liquid Stool  (Type 6 or 7 on the Bristol Stool Chart)

 

A.  Fresh liquid or soft stool  in a sterile container  WITHOUT PRESERVATIVE.

  1. Supplies -  Sterile and leak-proof container.
  2. Volume - Walnut size or 2 mL
  3. Collection Instructions:
    • Collect fresh, unpreserved liquid or soft stool.
    • If specimen is collected in a bedpan or collection “hat”, patient should urinate into another container before collecting stool specimen.
    • Transfer stool to a sterile, leak proof container. Those portions of stool containing blood and mucus are especially significant and should be selected.
    • Label container with patient’s legal name, date, collection time and initials of the collector.
    • Refrigerate after collection.
    • Transport to the laboratory as soon as possible.
  4. Stability - up to 72 hours at 2 - 8° C

 

B.  Liquid or soft stool  in Cary Blair (CB) Transport Media  WITH PRESERVATIVE.

  1. Supplies - Cary Blair Transport Medium
  2. Volume - Fill CB Transport Media with stool until container contents rise to the red line.
  3. Collection Instructions:
    • Collect as per Section A instructions above for fresh liquid or soft stool.
    • Transfer stool into CB Transport Media. Those portions of stool containing blood and mucus are especially significant and should be selected.
    • Tighten cap and mix well.
    • Label container with patient legal name, date, collection time and initials of the collector.
    • Refrigerate after collection.
    • Transport to the laboratory as soon as possible.
  4. Stability - up to 72 hours at 2 - 8° C

SPECIMEN REJECTION

Reject Due To:

  • Stool at ambient temperature > 2 hours
  • Stool refrigerated > 3 days
  • Specimens submitted in formalin r polyvinyl alcohol
  • Rectal swab
  • Contaminated with toilet water or urine
  • Specimens from infants less than 2 years of age
  • Specimens submitted in a diaper

MAXIMUM LABORATORY TIME

24 to 48 hours

STAT available 24/7

DAY(S) TEST SET UP

Daily

REFERENCE RANGES

Negative

TEST CLASSIFICATION AND CPT CODING

87324

87449