Test Code N.CDIFF C. DIFFICILE TOXIN A/B (Screen)
Methodology
TECHLAB rapid membrane enzyme immunoassay for the detection of Clostridioides (Clostridium) difficile toxins A and B in stool specimens. This test is useful for the rapid diagnosis of Clostridium difficile associated diarrhea (CDAB) and pseudomembranous colitis.
Specimen Requirements
Soft or Liquid Stool (Type 6 or 7 on the Bristol Stool Chart)
A. Fresh liquid or soft stool in a sterile container WITHOUT PRESERVATIVE.
- Supplies - Sterile and leak-proof container.
- Volume - Walnut size or 2 mL
- Collection Instructions:
- Collect fresh, unpreserved liquid or soft stool.
- If specimen is collected in a bedpan or collection “hat”, patient should urinate into another container before collecting stool specimen.
- Transfer stool to a sterile, leak proof container. Those portions of stool containing blood and mucus are especially significant and should be selected.
- Label container with patient’s legal name, date, collection time and initials of the collector.
- Refrigerate after collection.
- Transport to the laboratory as soon as possible.
- Stability - up to 72 hours at 2 - 8° C
B. Liquid or soft stool in Cary Blair (CB) Transport Media WITH PRESERVATIVE.
- Supplies - Cary Blair Transport Medium
- Volume - Fill CB Transport Media with stool until container contents rise to the red line.
- Collection Instructions:
- Collect as per Section A instructions above for fresh liquid or soft stool.
- Transfer stool into CB Transport Media. Those portions of stool containing blood and mucus are especially significant and should be selected.
- Tighten cap and mix well.
- Label container with patient legal name, date, collection time and initials of the collector.
- Refrigerate after collection.
- Transport to the laboratory as soon as possible.
- Stability - up to 72 hours at 2 - 8° C
SPECIMEN REJECTION
Reject Due To:
- Stool at ambient temperature > 2 hours
- Stool refrigerated > 3 days
- Specimens submitted in formalin r polyvinyl alcohol
- Rectal swab
- Contaminated with toilet water or urine
- Specimens from infants less than 2 years of age
- Specimens submitted in a diaper
MAXIMUM LABORATORY TIME
24 to 48 hours
STAT available 24/7
DAY(S) TEST SET UP
Daily
REFERENCE RANGES
Negative
TEST CLASSIFICATION AND CPT CODING
87324
87449