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Test Code N.VITD VITAMIN D (25-OH), CALCIFEDIOL, D3

Methodology

Automated Chemistry Analyzer

Specimen Requirements

Serum or Plasma

Container: SST tube or Green top Lithium heparin

Specimen Collection: 1 mL (minimum 0.5 mL)

  1. Allow specimen to clot completely if  a serum tube (SST) was used.
  2. Separate serum (SST) or plasma (Lithium Heparin) from cells within 24 hours and transport refrigerated. If red top tube is collected, separate serum from cells within 2 hours and place in separate plastic tube.
  3. Properly label tube with patient's name, DOB, requisition number and type of tube, i.e. GREEN.

Specimen Transport/Stability

Refrigerated: 12 days

Specimen Rejection

1. Exceed stability requirements

2. Unlabeled specimen

Maximum Laboratory Time

24 - 48 hours

Reference Ranges

Vitamin D3       ng/mL
Suggests deficiency of 25-OH Vitamin D      <  20
Suggests relative insufficiency of 25-OH Vitamin D      <  29
Suggests sufficient level of 25-OH Vitamin D       >  30

 

Day(s) Test Set Up

Daily

Test Classification and CPT Coding

82306