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Test Code N.VITD VITAMIN D (25-OH), CALCIFEDIOL, D3

Methodology

Automated Chemistry Analyzer

Specimen Requirements

Specimen Type: Serum or Plasma 
Container/Tube: SST or Lithium Heparin 

Acceptable: Red top 
Specimen Volume: 1 mL (minimum 0.5) 
Collection Instructions:  

  1. Allow specimen to clot completely if a serum tube (SST) was used. 
  2. Separate serum (SST) or plasma (Lithium Heparin) from cells within 24 hours and transport refrigerated. If red top tube is collected, separate serum from cells within 2 hours and place in separate plastic tube. 
  3. Properly label tube with patient's name, DOB, requisition number and type of tube, i.e. LiHep or NaHep etc. 

Specimen Rejection

1. Exceeds stability requirements

2. Gross hemolysis

3. Unlabeled/mislabeled specimen

Maximum Laboratory Time

24 - 48 hours

Specimen Transport/Stability

Refrigerated: 6 days

Test Classification and CPT Coding

82306

Day(s) Test Set Up

Daily

Reference Ranges

Vitamin D3       ng/mL
Suggests deficiency of 25-OH Vitamin D      <  20
Suggests relative insufficiency of 25-OH Vitamin D      <  29
Suggests sufficient level of 25-OH Vitamin D       >  30