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Test Code N.FLUCOV FLU A/B, COVID-19 (PCR)

Methodology

Real-Time Polymerase Chain Reaction (PCR)

Specimen Requirements

Nasopharyngeal

Container: Universal Transport Media

Specimen Collection:

  1. Use a sterile flexible minitip flocked swab with a synthetic tip (e.g. Dacron, nylon, or rayon) and an aluminum or plastic shaft.
  2. Instruct patient to blow their nose. 
  3. Tilt patient's head back at a 70 degree angle.
  4. Insert swab into one nostril straight back and continue along the floor of the nasal passage for several cenitmeters until reaching nasopharynx.
  5. Rotate swab gently for 5-10 seconds to loosen epithelial cells.
  6. Remove swab and immediately insert into Universal Transport Media. Break swab shaft and leave swab in the tube. 

Alternate Specimens: Polyester or cotton swabs in M4 media.

Specimen Transport/Stability

Ambient: 24 hours

Refrigerated (2-8⁰C): 72 hours

Frozen (-70⁰C): 30 days

 

 

Specimen Rejection

1. Cotton or calcium alginate swabs.

2. Swabs with wood shafts. 

Maximum Laboratory Time

24 hours

Reference Ranges

Influenza A PCR     Not Detected
Influenza B PCR     Not Detected
COVID-19     Not Detected

 

Day(s) Test Set Up

Daily

Test Classification and CPT Coding

87631