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HelpTest Code N.B12 VITAMIN B12 ASSAY
Methodology
Automated Chemistry Analyzer
Specimen Requirements
Specimen Type: Serum or Plasma
Container/Tube: SST or Lithium Heparin
Acceptable: Red top
Specimen Volume: 1 mL (minimum 0.5)
Collection Instructions:
- Allow specimen to clot completely if a serum tube (SST) was used.
- Separate serum (SST) or plasma (Lithium Heparin) from cells within 24 hours and transport refrigerated. If red top tube is collected, separate serum from cells within 2 hours and place in separate plastic tube.
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Properly label tube with patient's name, DOB, requisition number and type of tube, i.e. LiHep or NaHep etc.
Specimen Rejection
- Exceeds stability
- Hemolysis
- Unlabeled/mislabeled specimen
Maximum Laboratory Time
24 - 48 hours
Specimen Transport/Stability
Refrigerated: 6 days
If there will be delay in transport, centrifuge specimen according to instructions above and transfer to properly labeled aliquot container.
Test Classification and CPT Coding
82607
Day(s) Test Set Up
Daily
Reference Ranges
| Vitamin B12 | pg/mL |
| 239-931 |
Special Instructions
Use of heat-inactivated specimens or pooled specimens is not recommended.