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HelpTest Code N.B12 VITAMIN B12 ASSAY
Methodology
Automated Chemistry Analyzer
Specimen Requirements
Specimen Type: Serum or Plasma
Container/Tube: SST or Lithium Heparin
Acceptable: Red top
Specimen Volume: 1 mL (minimum 0.5)
Collection Instructions:
- Allow specimen to clot completely if a serum tube (SST) was used.
- Separate serum (SST) or plasma (Lithium Heparin) from cells within 24 hours and transport refrigerated. If red top tube is collected, separate serum from cells within 2 hours and place in separate plastic tube.
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Properly label tube with patient's name, DOB, requisition number and type of tube, i.e. LiHep or NaHep etc.
Specimen Transport/Stability
Refrigerated: 6 days
If there will be delay in transport, centrifuge specimen according to instructions above and transfer to properly labeled aliquot container.
Specimen Rejection
- Exceeds stability
- Hemolysis
- Unlabeled/mislabeled specimen
Maximum Laboratory Time
24 - 48 hours
Reference Ranges
| Vitamin B12 | pg/mL |
| 213 - 816 |
Day(s) Test Set Up
Daily
Test Classification and CPT Coding
82607
Special Instructions
Use of heat-inactivated specimens or pooled specimens is not recommended.