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Test Code N.B12 VITAMIN B12 ASSAY

Important Note

NO HEMOLYSIS

Methodology

Automated Chemistry Analyzer (ALINITY)

Specimen Requirements

Specimen Type: Serum or Plasma 
Container/Tube: SST or Lithium Heparin 
Specimen Volume: 1 mL (minimum 0.5) 
Collection Instructions:  

  1. Allow specimen to clot completely if a serum tube (SST) was used. 

  1. Separate serum (SST) or plasma (Lithium Heparin) from cells within 24 hours and transport refrigerated. If red top tube is collected, separate serum from cells within 2 hours and place in separate plastic tube. 

  1. Properly label tube with patient's name, DOB, requisition number and type of tube, i.e. LiHep or NaHep etc.  

Specimen Transport/Stability

Refrigerated: 6 days 

Note: Test can be run off the primary tube or an aliquot container, if sending specimens from other healthcare facilities please aliquot into separate tube. 

 

Specimen Rejection

1. Hemolyzed

2. Exceeding stability requirements

3. Unlabeled/mislabeled specimen

4. Heat in-activated specimen

Maximum Laboratory Time

24 - 48 hours

Reference Ranges

Vitamin B12       pg/mL
      213 - 816

 

Day(s) Test Set Up

Daily

Test Classification and CPT Coding

82607