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Test Code FLT FLT3 Mutation Analysis, Varies

Reporting Name

FLT3 Mutation Analysis, V

Useful For

Prognostic indication for some patients with acute myeloid leukemia

 

This test should not be used to monitor residual disease following treatment.

Method Name

Polymerase Chain Reaction (PCR)/Capillary Electrophoresis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Ordering Guidance


This test is intended to be used as a prognostic test at diagnosis and should not be used to monitor residual disease following treatment.



Shipping Instructions


Specimen must arrive within 7 days of collection.



Necessary Information


The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date and time of collection

4. Specimen source



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA) or yellow top (ACD)

Specimen Volume: 3 mL

Collections Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube. Do not aliquot.

3. Label specimen as blood.

Specimen Stability Information: Ambient (preferred)/Refrigerate

 

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA) or yellow top (ACD)

Specimen Volume: 2 mL

Collections Instructions:

1. Invert several times to mix bone marrow.

2. Send specimens in original tube. Do not aliquot.

3. Label specimen as bone marrow.

Specimen Stability Information: Ambient (preferred)/Refrigerate

 

Specimen Type: Extracted DNA from blood or bone marrow

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions:

1. Label specimen as extracted DNA from blood or bone marrow

2. Indicate volume and concentration of DNA on the label.

Specimen Stability Information: Frozen (preferred)/Refrigerate/Ambient


Reject Due To

Gross hemolysis Reject
Bone marrow biopsies, slides, paraffin shavings
Moderately to severely clotted		 Reject

Specimen Minimum Volume

Blood, Bone Marrow: 1 mL
Extracted DNA: 50 mcL at 20 ng/mcL concentration

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies 7 days

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Saturday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81245-FLT3 (fms-related tyrosine kinase) (eg, acute myeloid leukemia), gene analysis, internal tandem duplication (ITD) variants (ie, exons 14, 15)

81246-FLT3 (fms-related tyrosine kinase 3) (eg, acute myeloid leukemia), gene analysis; tyrosine kinase domain (TKD) variants (eg, D835, I836)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FLT FLT3 Mutation Analysis, V 79210-1

 

Result ID Test Result Name Result LOINC Value
MP009 Specimen Type 31208-2
41935 FLT3 Result 79210-1
19236 Final Diagnosis: 34574-4

Report Available

3 to 6 days

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Secondary ID

19739